Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Applied Biosystems™ TaqMan™ Exogenous Internal Positive Control Reagents
TaqMan™ Exogenous Internal Positive Control Reagents contain a pre-optimized internal positive control (IPC) with pre-designed primers and TaqMan™ probe.
Applied Biosystems™ ViralSEQ™ dPCR Lentivirus Proviral Titer Kit
The Applied Biosystems ViralSEQ dPCR Lentivirus Proviral DNA Titer Kit provides a wide dynamic range and high sensitivity for accurate detection of integrated provirus copies.
Applied Biosystems™ TaqPath™ COVID-19, Flu A, Flu B, RSV Select Panel
A multiplex real-time PCR solution that contains the assay, master mix, and controls needed for the simultaneous detection of RNA from COVID-19, influenza A, influenza B, and RSV in extracted nasopharyngeal swab specimens.
Thermo Scientific™ SureTect™ Staphylococcus aureus PCR Assay
Rapidly detect Staphylococcus aureus in food samples with the Thermo Scientific™ SureTect™ Staphylococcus aureus PCR Assay.
Applied Biosystems™ TaqMan™ Advanced miRNA Human Endogenous Controls 96-well Plate, standard
The Applied Biosystems TaqMan Advanced Human Endogenous Controls 96-Well Plate, Standard, provides the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well standard plate format.
Applied Biosystems™ ViralSEQ™ Mouse Minute Virus (MMV) Detection System
The Applied Biosystems ViralSEQ Mouse Minute Virus (MMV) Detection Assay is part of an integrated DNA purification and real-time PCR-based system for rapid, sensitive detection of MMV from a wide range of sample types.
Applied Biosystems™ TaqMan Fast Advanced Master Mix Gene Expression Bundle with MicroAmp Fast Plates and MicroAmp Seals PROMO
TaqMan Fast Advanced Master Mix delivers better sensitivity, greater specificity, and faster results.
Applied Biosystems™ Path-ID™ Multiplex One-Step Kit
The Path-ID™ Multiplex One-Step RT-PCR Kit is designed for multiplex, quantitative, reverse transcription PCR (qRT-PCR).
Applied Biosystems™ TaqMan™ PreAmp Master Mix Kit
TaqMan™ PreAmp Master Mix Kit contains TaqMan™ PreAmp Master Mix and TaqMan™ Gene Expression Master Mix.
Applied Biosystems™ TaqMan Fast Advanced Master Mix Gene Expression Bundle PROMO
TaqMan Fast Advanced Master Mix delivers better sensitivity, greater specificity, and faster results.
Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (5 mL sample prep, 1 mL PCR)
Go from Biological Sample to Genotyping Results in less than one hour.
Applied Biosystems™ PureQuant CD3+ T Cell Assay
The Applied Biosystems PureQuant assays are a family of assays that accurately identify and quantify specific immune cell types in a mixed population of cells. These assays are TaqMan qPCR-based and measure genomic DNA methylation at specific sites that serve as a unique identifier. The assays include standards and controls and each 96-well plate can accommodate up to seven test samples.
Applied Biosystems™ ViralSEQ™ Lentivirus Proviral DNA Titer Kits
A qPCR assay for the quantitation of integrated proviral DNA in transduced cells. Proviral copy number can be used to calculate infectious lentiviral titers (TU/mL) and subsequently, the volume of vector supernatant needed to transduce cells at the desired multiplicity of infection (MOI).
Thermo Scientific™ SureTect™ Salmonella species, Typhimurium and Enteritidis Multiplex Flex Assay
Rapidly detect and differentiate Salmonella species, Salmonella ser. Typhimurium, and Salmonella ser. Enteritidis in a single test with the SureTect Salmonella species, Typhimurium and Enteritidis Multiplex Flex Assay.