Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Thermo Scientific™ SureTect™ Staphylococcus aureus PCR Assay
Rapidly detect Staphylococcus aureus in food samples with the Thermo Scientific™ SureTect™ Staphylococcus aureus PCR Assay.
Applied Biosystems™ resDNASEQ™ Quantitative MDCK DNA Kits
The resDNASEQ™ Quantitative MDCK DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from MDCK (canine kidney) cells, an expression system commonly used for the production of vaccines.
Applied Biosystems™ ViralSEQ™ Mouse Minute Virus (MMV) Detection System
The Applied Biosystems ViralSEQ Mouse Minute Virus (MMV) Detection Assay is part of an integrated DNA purification and real-time PCR-based system for rapid, sensitive detection of MMV from a wide range of sample types.
Applied Biosystems™ resDNASEQ™ Quantitative E1A DNA Fragment Length Kits
The resDNASEQ Quantitative E1A DNA Fragment Length Kit is a quantitative PCR (qPCR)-based system that measures and quantitates the fragment lengths of residual host-cell DNA by targeting the E1A gene in HEK 293 cell lines.
Applied Biosystems™ TrueMark™ Amplification Control I (5 x 107 copies/μL)
The TrueMark Amplification Control I for TrueMark plates contains a linearized multi-target plasmid pool with microbial target sequences used on TrueMark infectious disease research panels.
Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (5 mL sample prep, 1 mL PCR)
Go from Biological Sample to Genotyping Results in less than one hour.
Applied Biosystems™ ViralSEQ™ Quantitative Sf-Rhabdovirus Kits
The Applied Biosystems ViralSEQ Quantitative Sf-Rhabdovirus Kit is a two-step RT-qPCR assay for the detection of Sf-rhabdovirus in recombinant products manufactured in insect cell lines using baculovirus expression systems.
Applied Biosystems™ PureQuant CD3+ T Cell Assay
The Applied Biosystems PureQuant assays are a family of assays that accurately identify and quantify specific immune cell types in a mixed population of cells. These assays are TaqMan qPCR-based and measure genomic DNA methylation at specific sites that serve as a unique identifier. The assays include standards and controls and each 96-well plate can accommodate up to seven test samples.
Applied Biosystems™ TaqMan Fast Advanced Master Mix Gene Expression Bundle PROMO
TaqMan Fast Advanced Master Mix delivers better sensitivity, greater specificity, and faster results.
Thermo Scientific™ SureTect™ Escherichia coli O157:H7 and STEC Screening PCR Assay
Rapidly detect E. coli O157:H7, stx, eae, in food samples in as little as 10 hours with highly-specific, simple to use real-time PCR Thermo Scientific™ SureTect™ Escherichia coli O157:H7 and STEC Screening PCR Assay.
Thermo Scientific™ SureTect™ Salmonella species, Typhimurium and Enteritidis Multiplex Flex Assay
Rapidly detect and differentiate Salmonella species, Salmonella ser. Typhimurium, and Salmonella ser. Enteritidis in a single test with the SureTect Salmonella species, Typhimurium and Enteritidis Multiplex Flex Assay.
Applied Biosystems™ TaqMan™ PreAmp Master Mix Kit
TaqMan™ PreAmp Master Mix Kit contains TaqMan™ PreAmp Master Mix and TaqMan™ Gene Expression Master Mix.
Applied Biosystems™ resDNASEQ™ Quantitative Pichia pastoris DNA Kits
The resDNASEQ™ Quantitative Pichia pastoris DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Pichia pastoris cells.
Applied Biosystems™ TrueMark™ Enteric Bacterial Select Panel II Combo Kit
The TrueMark Enteric Bacterial Select Panel II Combo Kit includes the TrueMark Enteric Bacterial Select Panel II and TrueMark Infectious Disease 1‐Step Multiplex Master Mix for simplified ordering.